S1-Tag antibody

Catalog No. ABIN1573920

This Rabbit Polyclonal antibody specifically detects S1-Tag in WB and ELISA.

Quick Overview for S1-Tag antibody (ABIN1573920)

Target: S1-Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-S1-Tag Antibody

Specificity: This Antibody recognizes both N-terminal and C-terminal S1 tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: S1 epitope tag peptide NANNPDWDF conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit:For quick results, ONE-HOUR WesternTM Kit is recommendet. 20 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for S1-Tag

Target: S1-Tag

Target Type: Tag

Background: S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems.The Rabbit Anti-S1-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.