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Cyclin D1 antibody (pThr286)

Name: Cyclin D1 antibody (pThr286)
SKU: ABIN1575927
Price: 791.54 USD
Availability: InStock

CCND1 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using Cyclin D1 (Phospho-Thr286) antibody. (Cyclin D1 antibody (pThr286))

1 image

Catalog No. ABIN1575927

Datasheet as PDF

Target See all Cyclin D1 (CCND1) Antibodies

Cyclin D1 (CCND1)
Binding Specificity
21

16

16

14

12

9

9

8

7

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr286
Reactivity
208

114

108

40

20

17

16

12

9

8

3

3

3

3

2

2

2

1

1

1

1
Human, Mouse, Rat
Host
177

53

1
Rabbit
Compatible Secondaries
Clonality
165

64
Polyclonal
Conjugate
117

21

17

7

5

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This Cyclin D1 antibody is un-conjugated
Application
182

74

61

60

39

28

28

27

24

20

19

11

6

5

3

2

2

2

1

1

1
Western Blotting (WB), ELISA

Specificity

Epitopes for Species: H:T286,M:T286,R:T286

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Cyclin D1 around the phosphorylation site of threonine 286 (A-C-TP-P-T).

Isotype

IgG

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Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Storage

-20 °C

Storage Comment

Store at -20°C / 1 year

Expiry Date

12 months
Target

Cyclin D1 (CCND1)

Alternative Name

Cyclin D1 (CCND1 Products)

UniProt

P24385

Pathways

PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling