ALDOC antibody (C-Term)

Catalog No. ABIN1580409

This anti-ALDOC antibody is a Mouse Monoclonal antibody detecting ALDOC in WB, IHC, IF and ICC. Suitable for Human, Mouse, Rat, Cow, Pig and Mammalian.

Quick Overview for ALDOC antibody (C-Term) (ABIN1580409)

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Reactivity: Human, Mouse, Rat, Cow, Pig, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALDOC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1A1

Product Details anti-ALDOC Antibody

Binding Specificity: C-Term

Purification: affinity purified antibody

Isotype: IgG1

Application Details

Application Notes: The antibody solution can be used at dilutions 1:1,000-2,000 for immunofluorescence. For western blots try at 1:2,000-5,000. A suitable control tissue is brain cell lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for ALDOC

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Alternative Name: Aldolase C

Background: Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene.Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors. In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons, aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific. MCA-1A1 was raised against C-terminal 23 amino acids of the human aldolase C protein, the sequence is KYEGSGEDGGAAAQSLYIANHAY. The HGNC name for this protein is ALDOC.