NEFL antibody

Catalog No. ABIN1580446

This Mouse Monoclonal antibody specifically detects NEFL in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Rat, Mouse, Pig, Cow and Mammalian.

Quick Overview for NEFL antibody (ABIN1580446)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Mouse, Pig, Cow, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: DA2

Product Details anti-NEFL Antibody

Purification: conc. tissue culture supernatant

Isotype: IgG1

Application Details

Application Notes: For immunofluorescence, use MCA-DA2 diluted 1/500. For western blots try MCA-DA2 diluted 1/10,000 to 1:20,000.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament NF-L

Background: Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease. This antibody has been sold by Chemicon and Millipore for many years under the catalog number Mab1615, and has been used in numerous publications. The same antibody is also marketed, more expensively, by Abcam, Novus and many other companies.