VEGF-Apan (Peptide) (N-Term) antibody

Catalog No. ABIN1589724

The Rabbit Polyclonal anti-VEGF-Apan (Peptide) antibody (ABIN1589724) specifically detects VEGF-Apan (Peptide) in IP and WB. The antibody is reactive with Human samples.

Quick Overview for VEGF-Apan (Peptide) (N-Term) antibody (ABIN1589724)

Target: VEGF-Apan (Peptide)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Purpose: VEGF-Apan (Peptide) antibody

Specificity: N-terminal peptide (20aa) of native human VEGF165

Characteristics: Chromosomal location: 6p12
Produced from sera of rabbits immunized with a highly purified N-terminal peptide of human VEGF-A.

Purification: Anti-VEGF-A was purified by affinity chromatography with immobilized Protein A.

Immunogen: N-terminal peptide (20aa) of native human VEGF165

Isotype: IgG

Application Details

Application Notes: IP: Use 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details

Target: VEGF-Apan (Peptide)

Background: VEGFA, VPF, VEGF, MVCD1,Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Different isoforms can be generated by differential splicing (e.g. VEGF165). All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53 % amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF-A with high affinity. In vitro, VEGF is a potent endothelial cell mitogen. The antibody is against the N-terminus and will recognize all VEGF-A isoforms.

Gene ID: 7422

NCBI Accession: NM_001171626, NP_001165097

UniProt: P15692