PLGF antibody (N-Term)

Catalog No. ABIN1589873

The Rabbit Polyclonal anti-PLGF antibody has been validated for WB, ELISA and IF. It is suitable to detect PLGF in samples from Human.

Quick Overview for PLGF antibody (N-Term) (ABIN1589873)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PLGF Antibody

Binding Specificity: N-Term

Purpose: PlGF (Peptide) antibody

Specificity: N-terminal peptide (20aa) of native human PlGF

Characteristics: Chromosomal location: 2p21-p16
Produced from sera of rabbits immunized with a highly purified N-terminal peptide of human PlGF (placenta growth factor).

Purification: Anti-PlGF was purified by affinity chromatography with immobilized Protein A.

Immunogen: N-terminal peptide (20aa) of native human PlGF

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 2-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: PlGF

Background: PlGF, placental growth factor,Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF1), 152 (PlGF2), and 203 (PlGF3) amino acids (aa) respectively. Only PlGF2 contains a highly basic heparinbinding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF2 has been identified. Human PlGF1 shares 56 % , 55 %, 74 % and 95 % aa identity with the appropriate isoform of mouse, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation, this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PlGF (especially PlGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF2, but not PLGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.

Gene ID: 5281

NCBI Accession: NM_001207012, NP_001193941

UniProt: P49763

Pathways: VEGFR1 Specific Signals