CEACAM1 antibody (deltaN-Term)

Catalog No. ABIN1589889

Product Details anti-CEACAM1 Antibody

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Binding Specificity: deltaN-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEACAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Purpose: CEACAM-1 (dN) antibody

Specificity: Recombinant human sCEA-1 (ΔN)

Characteristics: Chromosomal location: 19q13.2
Produced from sera of rabbits immunized with highly pure recombinant human soluble CEACAM-1 (ΔN) (Carcinoembryonic antigen-related cell adhesion molecule-1, SwissProt: #P13688) as the immunizing antigen.

Purification: Anti-human soluble CEACAM-1 was purified by affinity chromatography with immobilized Protein A.

Immunogen: Recombinant human sCEA-1 (deltaN)

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 2-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CEACAM-1 (CEACAM1 Products)

Background: CEACAM1, BGP, BGP1, BGPI, CD66a,Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1, also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa's 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57 % aa identical to human CEACAM1, in the V-type Ig-like domain, they are 58 % and 56 % aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.

Gene ID: 634

NCBI Accession: NM_001205344, NP_001192273

UniProt: P13688