CEACAM1 antibody

Catalog No. ABIN108725

The Mouse Monoclonal anti-CEACAM1 antibody is suitable to detect CEACAM1 in samples from Human. It has been validated for FACS, IHC (fro) and cELISA.

Quick Overview for CEACAM1 antibody (ABIN108725)

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cell-ELISA (cELISA)

Clone: GM-8G5

Product Details anti-CEACAM1 Antibody

Specificity: GM8G5 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells as well as the naturally expressed protein (Fig. 2, kindly provided by Dr. R. Riesenberg, Labor für Tumorimmunologie, Urologische Klinik, Klinikum Grossh

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding the extracellular region of human CEACAM1-A2

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400
Immunohistology: 10 µg/mL (on cryosections)
For each application a titration should be performed to determine the optimal concentration.

Comment:

Synonyms: CD66a, BGP, biliary glycoprotein I

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CEACAM1

Background: CEACAM1 (BGP) belongs to the carcinoembryonic antigen (CEA) gene family. CEACAM1 expression is downregulated in many tumors indicating a tumor-suppressive function. GM8G5 was generated by genetic immunisation and can be used to distinguish CEACAM1 from all other CEACAMs.

UniProt: P13688