Podoplanin antibody (Biotin)

Catalog No. ABIN1589928

The Biotin-conjugated Hamster Monoclonal anti-Podoplanin antibody (Clone RTD4E10) (ABIN1589928) specifically detects Podoplanin in WB and IHC. The antibody is reactive with Mouse samples.

Quick Overview for Podoplanin antibody (Biotin) (ABIN1589928)

Target: Podoplanin (PDPN)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RTD4E10

Product Details anti-Podoplanin Antibody (Biotin)

Purpose: Podoplanin antibody

Specificity: Murine stromal cell line

Characteristics: Chromosomal location: 4 E1, 4
Monoclonal antibodies were produced by immunizing Syrian hamsters with a murine thymic stromal cell line.

Purification: This antibody recognizes mouse Podoplanin. Hamster IgG antibody from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogen: Murine stromal cell line

Isotype: IgG

Application Details

Application Notes: Western Blot: use at 2-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Background: Pdpn, T1a, Gp38, OTS-8, T1alpha, RANDAM-2, T1-alpha,Podoplanin, also known as T1 alpha (T1α), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5 % and 29 % amino acid sequence homology with rat and human Podoplanin, respectively.

Gene ID: 14726

NCBI Accession: NM_010329, NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport