KIT Ligand antibody

Catalog No. ABIN1589938

This anti-KIT Ligand antibody is a Rabbit Polyclonal antibody detecting KIT Ligand in WB. Suitable for Mouse.

Quick Overview for KIT Ligand antibody (ABIN1589938)

Target: KIT Ligand (KITLG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIT Ligand Antibody

Purpose: SCF antibody

Specificity: Recombinant mouse SCF

Characteristics: Chromosomal location: 10 D1, 10 57.0 cM

Purification: Protein A purified

Immunogen: Recombinant mouse SCF (ABIN1589733)

Isotype: IgG

Application Details

Application Notes: Western Blot: use at 2-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: SCF

Background: Kitl, Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, Kitlg, contrasted,Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxta-membrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/ckit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial

Gene ID: 17311

NCBI Accession: NM_013598, NP_038626

UniProt: P20826

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway