CD19 antibody (AA 422-433)

Catalog No. ABIN1589984

The Goat Polyclonal anti-CD19 antibody has been validated for WB and ELISA. It is suitable to detect CD19 in samples from Human.

Quick Overview for CD19 antibody (AA 422-433) (ABIN1589984)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD19 Antibody

Binding Specificity: AA 422-433

Purpose: CD19 (aa422-433)

Sequence: ENDSNLGQDQ LS

Specificity: This antibody is expected to recognize both reported isoforms (NP_001171569.1, NP_001761.3).

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1 kDa according to NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 μg/mL. Primary incub

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: CD19, CD19 Molecule, B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19

Gene ID: 930

NCBI Accession: NP_001171569, NP_001761

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway