CLOCK antibody (AA 458-472)

Catalog No. ABIN1589996

The Goat Polyclonal anti-CLOCK antibody has been validated for WB and ELISA. It is suitable to detect CLOCK in samples from Mouse and Rat.

Quick Overview for CLOCK antibody (AA 458-472) (ABIN1589996)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CLOCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CLOCK Antibody

Binding Specificity: AA 458-472

Purpose: CLOCK (aa458-472)

Sequence: DTSTPPRQHL PAHEK

Specificity: Reported variants represent identical protein: NP_004889.1, NP_001254772.1

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.4 kDa according to Mouse NP_031741.1 and 97.0 kDa according to Rat NP_068628.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Alternative Name: CLOCK

Background: CLOCK, clock homolog (mouse), KAT13D, bHLHe8, circadian locomoter output cycles kaput protein, circadian locomoter output cycles protein kaput, class E basic helix-loop-helix protein 8

Gene ID: 9575, 12753

NCBI Accession: NP_004889

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism