TNNT3 antibody (C-Term)

Catalog No. ABIN1590029

The Goat Polyclonal anti-TNNT3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect TNNT3 in samples from Human, Mouse and Rat.

Quick Overview for TNNT3 antibody (C-Term) (ABIN1590029)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TNNT3 Antibody

Binding Specificity: C-Term

Purpose: TNNT3

Sequence: TTLRSRIDQA QKHSK

Specificity: This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1, NP_001036246.1, NP_001036247.1). Reported variants represent identical protein: NP_001284575.1, NP_001036247.1

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8 μg/mL.

Western Blot: Approx 35 kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6 kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: TNNT3

Background: TNNT3, troponin T type 3 (skeletal, fast), TNTF, beta TnTF, beta-TnTF, fTnT, fast skeletal muscle troponin T, troponin T, fast skeletal muscle, troponin-T3, skeletal, fast

Gene ID: 7140, 21957, 24838

NCBI Accession: NP_006748, NP_001036246, NP_001036247