RFX5 antibody (Internal Region)

Catalog No. ABIN1590073

This Goat Polyclonal antibody specifically detects RFX5 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for RFX5 antibody (Internal Region) (ABIN1590073)

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RFX5 Antibody

Binding Specificity: Internal Region

Purpose: RFX5

Sequence: TGDKSSEPST LSNE

Specificity: Reported variants represent identical protein: NP_000440.1, NP_001020774.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3 kDa according to NP_000440.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5

Background: RFX5, regulatory factor X, 5 (influences HLA class II expression), OTTHUMP00000196318, regulatory factor X, 5

Gene ID: 5993, 53970, 310659

NCBI Accession: NP_000440