DVL1 antibody (Internal Region)

Catalog No. ABIN1590089

The Goat Polyclonal anti-DVL1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect DVL1 in samples from Human and Mouse.

Quick Overview for DVL1 antibody (Internal Region) (ABIN1590089)

Target: DVL1 (Dishevelled Segment Polarity Protein 1 (DVL1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DVL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-DVL1 Antibody

Binding Specificity: Internal Region

Purpose: Dvl1 (mouse)

Sequence: PQLEEAPLTV KSD

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 75.4 kDa according to NP_034221.3). An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DVL1

Target: DVL1 (Dishevelled Segment Polarity Protein 1 (DVL1))

Alternative Name: Dvl1

Background: Dvl1, dishevelled, dsh homolog 1 (Drosophila), RP23-242K15.15-003, Dvl, mKIAA4029, DSH homolog 1, dishevelled-1, segment polarity protein dishevelled homolog DVL-1

Gene ID: 13542, 83721

NCBI Accession: NP_034221, NP_001343310

Pathways: WNT Signaling, Synaptic Membrane, Skeletal Muscle Fiber Development