Beta-Phosphoglucomutase antibody (Biotin)

Catalog No. ABIN1607649

Supplier Product No.: 200-106-237s

This anti-Beta-Phosphoglucomutase antibody is a Goat Polyclonal antibody detecting Beta-Phosphoglucomutase in WB and ELISA. Suitable for Lactococcus lacti.

Quick Overview for Beta-Phosphoglucomutase antibody (Biotin) (ABIN1607649)

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Reactivity: Lactococcus lacti

Host: Goat

Clonality: Polyclonal

Conjugate: This Beta-Phosphoglucomutase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Beta-Phosphoglucomutase Antibody (Biotin)

Supplier Product No.: 200-106-237s

Supplier: Rockland

Purpose: Beta-Phosphoglucomutase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Beta-Phosphoglucomutase [Lactococcus lacti].

Characteristics: Synonyms: goat anti-Beta-Phosphoglucomutase Antibody biotin Conjugation, biotin conjugated goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody

Purification: Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: beta Phosphoglucomutase [Lactococcus lacti]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Beta-Phosphoglucomutase Biotin has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Beta-Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 is suggested for this product.

Western Blot Dilution: 1:200 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Beta-Phosphoglucomutase

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Alternative Name: pgmB

Background: Background: Anti-Beta-Phosphoglucomutase antibody is located in the cytoplasm, working to catalyze the transference of a phosphoryl group on a glucose or lactose monomer from the 1' to the 6' position in the forward direction or 6' to the 1' position in the reverse direction. Anti-Beta-Phosphoglucomutase is essential to regulating the flow of carbohydrate intermediates in glycolysis as well as the formation of the sugar nucleotide UDP-glucose. Part of the HAD-like hydrolase superfamily, Anti-Beta-Phosphoglucomutase antibody is ideal for researchers interested in Metabolism research.

Gene ID: 1114041

UniProt: P71447