Beta-Phosphoglucomutase antibody (Biotin)

Catalog No. ABIN1607648

Product Details anti-Beta-Phosphoglucomutase Antibody (Biotin)

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Reactivity: Lactococcus lacti

Host: Goat

Clonality: Polyclonal

Conjugate: This Beta-Phosphoglucomutase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Beta-Phosphoglucomutase from other sources is unknown.

Purity: Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Beta-Phosphoglucomutase [Lactococcus lacti].

Endotoxin Level: Low Endotoxin : No

Immunogen: beta Phosphoglucomutase [Lactococcus lacti]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Beta-Phosphoglucomutase has been assayed against 1.0 µg of Beta-Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:50.000 is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Beta-Phosphoglucomutase

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Alternative Name: Beta-Phosphoglucomutase (Beta-Phosphoglucomutase Products)

Synonyms: ECK1312 antibody, JW1310 antibody, beta-phosphoglucomutase antibody, ycjU antibody, pgmB antibody, LMOf2365_2822 antibody, Exig_2478 antibody, Hore_00430 antibody, Tmath_0800 antibody, Aazo_0214 antibody, Spirs_3639 antibody, Closa_0065 antibody, Toce_1769 antibody, Tthe_1054 antibody, Lbys_0589 antibody, Celal_0979 antibody, Sgly_0722 antibody, Celly_2426 antibody, Spico_1537 antibody, Theth_1551 antibody

Background: Anti-Beta-Phosphoglucomutase antibody is located in the cytoplasm, working to catalyze the transference of a phosphoryl group on a glucose or lactose monomer from the 1’ to the 6’ position in the forward direction or 6’ to the 1’ position in the reverse direction. Anti-Beta-Phosphoglucomutase is essential to regulating the flow of carbohydrate intermediates in glycolysis as well as the formation of the sugar nucleotide UDP-glucose. Part of the HAD-like hydrolase superfamily, Anti-Beta-Phosphoglucomutase antibody is ideal for researchers interested in Metabolism research. Synonyms: Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody

Gene ID: 1114041

UniProt: P71447