GLN1 antibody (Biotin)

Catalog No. ABIN1607650

Supplier Product No.: 200-106-238-0100

The Goat Polyclonal anti-GLN1 antibody has been validated for WB, IP and ELISA. It is suitable to detect GLN1 in samples from Brevibacterium.

Quick Overview for GLN1 antibody (Biotin) (ABIN1607650)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Brevibacterium

Host: Goat

Clonality: Polyclonal

Conjugate: This GLN1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-GLN1 Antibody (Biotin)

Supplier Product No.: 200-106-238-0100

Supplier: Rockland

Purpose: Glutamine Synthetase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].

Characteristics: Synonyms: goat anti-Glutamine Synthetase Antibody biotin Conjugation, biotin Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase

Purification: Anti-Glutamine Synthetase (microbial) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glutamine Synthetase [Microbial]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 μg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Background: Background: Glutamine Synthetase is a key enzyme in the metabolism of nitrogen. Glutamine synthetase catalyzes an ATP-dependent condensation reaction between ammonia and glutamate to yield glutamine. Glutamine is a key builder of proteins as well as a vehicle to deliver nitrogen atoms to enzymes that build molecules dependent on nitrogen. Glutamine Synthetase from a microbial source is composed of twelve subunits that each house an active site. During the reaction of glutamine synthetase, the active sites bind ammonia and glutamate, as well as an ATP molecule to power the reaction. Negative feedback regulation is provided by the active sites ability to weakly bind other molecules and once their concentrations rise too high, the enzyme shuts off. Glutamine Synthetase has applications in neuroscience due to location in astrocytes within the brain and fluctuations in glutamine synthetase can detrimentally effect the astrocytes. Anti-Glutamine Synthetase (Microbial) Antibody is ideal for investigators in Molecular Biology, Neuroscience, and Enzymology.

Gene ID: 3345165

UniProt: Q79VE3

Pathways: Positive Regulation of Peptide Hormone Secretion