FGA antibody (Biotin)

Catalog No. ABIN1607652

Supplier Product No.: 200-106-240-0100

This Goat Polyclonal antibody specifically detects FGA in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for FGA antibody (Biotin) (ABIN1607652)

Target: FGA (Fibrinogen alpha Chain (FGA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FGA Antibody (Biotin)

Supplier Product No.: 200-106-240-0100

Supplier: Rockland

Purpose: Fibrinogen Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma].

Characteristics: Synonyms: goat anti-Fibrinogen Antibody biotin Conjugation, biotin Conjugated goat anti-Fibrinogen Antibody, FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody

Purification: Anti-Fibrinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Fibrinogen [Human Plasma]

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Fibrinogen Biotin antibody has been tested by ELISA, western blot, and immunohistochemistry. This product is assayed against 1.0 μg of Fibrinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50,000 to 1:250,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FGA

Target: FGA (Fibrinogen alpha Chain (FGA))

Alternative Name: FGA

Background: Background: Anti-Fibrinogen antibody has a dual function: yielding monomers that polymerize into fibrin and as a main protein of the blood coagulation system, it also functions as a cofactor in platelet aggregation. Fibrinogen clotting underlies pathogenesis of thromboembolism, MI, and thrombus formation. Fibrinogen activation is a major factor in the mechanism which causes inflammation, tumor growth and various additional diseases. These characteristics make Anti-Fibrinogen ideal for investigators interested in the fields of Cardiovascular or Metabolism research.

Gene ID: 2243

NCBI Accession: NP_000499

UniProt: P02671