FTH1 antibody (Biotin)

Catalog No. ABIN1607711

Supplier Product No.: 200-406-090-0100

This anti-FTH1 antibody is a Rabbit Polyclonal antibody detecting FTH1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for FTH1 antibody (Biotin) (ABIN1607711)

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FTH1 Antibody (Biotin)

Supplier Product No.: 200-406-090-0100

Supplier: Rockland

Purpose: Ferritin Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Characteristics: Synonyms: rabbit anti-Ferritin Antibody Biotin Conjugation, biotin conjugated rabbit anti-Ferritin Antibody, FTH, FTHL6

Purification: Anti-Ferritin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Ferritin Biotin antibody has been tested by western blot and is suitable for ELISA and Immunohistochemistry. Expect band at 21.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:200 - 1:1,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: FTH1

Background: Background: Ferritin Antibody detects human ferritin. Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The amount of ferritin stored reflects the amount of iron stored. The protein is produced by almost all living organisms, including algae, bacteria, higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is a globular protein complex consisting of 24 protein subunits and is the primary intracellular iron-storage protein in both prokaryotes and eukaryotes, keeping iron in a soluble and non-toxic form. Ferritin that is not combined with iron is called apoferritin. Anti-Ferritin antibody is ideal for investigators involved in enzyme and ubiquitin and UBL research.

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis