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Mutarotase antibody

Reactivity: Pig WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN1607744
Supplier Product No.: 200-4162s
  • Target
    Mutarotase
    Reactivity
    • 15
    • 6
    Pig
    Host
    • 21
    Rabbit
    Clonality
    • 21
    Polyclonal
    Conjugate
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 21
    • 13
    • 13
    • 6
    • 4
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    200-4162s
    Supplier
    Rockland
    Purpose
    Mutarotase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Mutarotase [Porcine Kidney].
    Characteristics
    Synonyms: rabbit anti-Mutarotase Antibody, Aldose 1 epimerase antibody, BLOCK25 protein antibody, Galactose mutarotase antibody, GALM antibody
    Purification
    Anti-Mutarotase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Mutarotase [Porcine Kidney]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Mutarotase Antibody has been assayed against 1.0 μg of Mutarotase [Porcine Kidney] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:22,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Mutarotase
    Background
    Background: Aldose 1-epimerase (Mutarotase) converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose. This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
    Gene ID
    399536
    UniProt
    Q9GKX6
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