Elastase antibody (Biotin)
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- Target See all Elastase Antibodies
- Elastase
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Elastase antibody is conjugated to Biotin
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Application
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-4668-0100
- Supplier
- Rockland
- Purpose
- Anti-Elastase Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].
- Characteristics
- Synonyms: rabbit anti-Elastase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody
- Purification
- Anti-Elastase (Rabbit) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Elastase [Porcine Pancreas]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product Elastase Primary Antibody
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- Application Notes
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Application Note: Anti-Elastase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Elastase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Elastase
- Abstract
- Elastase Products
- Background
- Background: Anti-Elastase antibody detects the Elastase protein. Elastase metabolizes elastin, an elastic fiber, which together with collagen, determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Elastase also has the important immunological role of breaking down Shigella virulence factors. This is accomplished through the cleavage of peptide bonds in the target proteins. The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic amino acids such as glycine, alanine, and valine. This antibody is suitable for researchers investigating biochemistry and stem cells.
- Gene ID
- 396766
- NCBI Accession
- NP_998988
- UniProt
- P00772
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