LYZ antibody (Biotin)

Catalog No. ABIN1607808

Supplier Product No.: 200-4672s

Product Details anti-LYZ Antibody (Biotin)

Target: LYZ (Lysozyme (LYZ))

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-4672s

Supplier: Rockland

Purpose: Lysozyme Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White].

Characteristics: Synonyms: rabbit anti-Lysozyme Antibody biotin Conjugation, biotin Conjugated rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White

Purification: Anti-Lysozyme Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 5.6 BAC molecules per Rabbit IgG molecule

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Lysozyme Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: LYZ (LYZ Products)

Background: Background: Anti-Lysozyme Antibody detects lysozyme. Lysozyme belongs to the glycoside hydrolases enzymes that damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk, and mucus. It is also present in cytoplasmic granules of the polymorphonuclear neutrophils. Large amounts of lysozyme can be found in egg white. Anti-Lysozyme Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Gene ID: 396218

NCBI Accession: NP_990612

UniProt: P00698