Thymic Stromal Lymphopoietin antibody (Biotin)

Catalog No. ABIN1607864

Supplier Product No.: 209-406-h48

The Rabbit Polyclonal anti-Thymic Stromal Lymphopoietin antibody has been validated for WB and ELISA. It is suitable to detect Thymic Stromal Lymphopoietin in samples from Human.

Quick Overview for Thymic Stromal Lymphopoietin antibody (Biotin) (ABIN1607864)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Thymic Stromal Lymphopoietin Antibody (Biotin)

Supplier Product No.: 209-406-h48

Supplier: Rockland

Purpose: TSLP Antibody Biotin Conjugated

Cross-Reactivity (Details): This antibody is specific for human TSLP protein.

Characteristics: Synonyms: rabbit anti-TSLP biotin conjugated antibody, rabbit anti-Thymic Stromal Lymphopoietin biotin conjugated antibody

Purification: Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: TSLP purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000-1:2000

ELISA Dilution: 1:20,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Background: Thymic Stromal Lymphopoietin (TSLP) is a hematopoietic cytokine. TSLP is mainly expressed in the heart, liver and prostate. TSLP sends signals through a heterodimeric receptor complex, containing the TSLP-R and the IL-7Rα chain to induce the release of T cell-attracting chemokines from monocytes. It can induce allergic inflammation by directly activating mast cells. It also promotes T helper type 2 (TH2) cell responses associated with immunity, including atopic dermatitis and asthma, and can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of dendritic cells derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues. Recombinant human TSLP is a non-glycosylated protein with a molecular weight of 15 kDa. Anti-TSLP antibody is ideal for researchers interested in cytokines and stem cell research.

Gene ID: 85480

NCBI Accession: NP_149024

UniProt: Q969D9