Transferrin antibody (Biotin)

Catalog No. ABIN1607870

Supplier Product No.: 209-4634-0100

This anti-Transferrin antibody is a Rabbit Polyclonal antibody detecting Transferrin in WB, ELISA and IHC. Suitable for Human.

Quick Overview for Transferrin antibody (Biotin) (ABIN1607870)

Target: Transferrin (TF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Transferrin Antibody (Biotin)

Supplier Product No.: 209-4634-0100

Supplier: Rockland

Cross-Reactivity (Details): Cross reactivities against Transferrin from other sources may occur but have not been specifically determined.

Purity: Anti-TRANSFERRIN Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum].

Endotoxin Level: Low Endotoxin : No

Immunogen: Transferrin [Human Serum]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-TRANSFERRIN Antibody, Biotin Conjugated has been tested for use in Immunoblotting, ELISA, immunohistochemistry, immunofluorescence microscopy as well as other antibody based assays. Optimal concentrations should be determined by the researcher.
ELISA Dilution: 1:20.000 - 1:150.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin

Background: Anti-Transferrin Antibody, Biotin Conjugated is specific for the transferrin protein. Transferrins are iron-binding blood plasma glycoproteins which regulate the level of free iron in biological fluids. When free of bound iron, transferrin is referred to as apotransferrin. Human transferrin is encoded by the TF gene and is mainly synthesized in the liver, but other sources, such as the brain produce it. The main role of transferrin is delivery of iron from absorption centers in the duodenum and macrophages to all tissues. Membrane receptors on erythroid precursors in the bone marrow avidly bind transferrin. Hereditary hemochromatosis and anemia are well establish disorders involving defects in transferrin transport defects. Anti-Transferrin Antibody, Biotin Conjugated is suitable for use in cardiovascular research as well as other basic research. Synonyms: Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody

Gene ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis