HDAC5 antibody (Internal Region)

Catalog No. ABIN1607888

Product Details anti-HDAC5 Antibody

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with HDAC5 from other sources has not been determined.

Purity: Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC5. A BLAST analysis was used to suggest cross-reactivity with Human based on 100 % sequence homology.

Endotoxin Level: Low Endotoxin : No

Immunogen: HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human HDAC5.
Immunogen Type: Peptide

Application Details

Application Notes: Anti-HDAC5 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 0.2 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.12 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is six (6) months from date of opening.

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5 (HDAC5 Products)

Synonyms: HD5 antibody, NY-CO-9 antibody, AI426555 antibody, Hdac4 antibody, mHDA1 antibody, mKIAA0600 antibody, ATHDA5 antibody, HDA5 antibody, MAF19.7 antibody, MAF19_7 antibody, histone deacetylase 5 antibody, histone deacetylase 5 antibody, HDAC5 antibody, Hdac5 antibody, HDA05 antibody, hdac5 antibody

Background: HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. Anti-HDAC5 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research. Synonyms: Antigen NY-CO-9, EC 3.5.1.98, FLJ90614, HD5, histone deacetylase 5, KIAA0600, NY-CO-9

Gene ID: 10014

NCBI Accession: NP_005465

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process