D1-Nt antibody

Catalog No. ABIN165361

This Rabbit Polyclonal antibody specifically detects D1-Nt in WB. It exhibits reactivity toward Arabidopsis, Cyanobacteria (Nostoc) and Nicotiana tabacum.

Quick Overview for D1-Nt antibody (ABIN165361)

Target: D1-Nt (PsbA)

Reactivity: Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-D1-Nt Antibody

Specificity: Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.

Immunogen: synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.

Isotype: IgG

Application Details

Application Notes: Western blot (1 : 2.000 for ECL)

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Target Details for D1-Nt

Target: D1-Nt (PsbA)

Background: D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).