The Rabbit Polyclonal anti-Thylakoid Proteins antibody (ABIN165369) specifically detects Thylakoid Proteins in WB.
The antibody is reactive with Arabidopsis, Pisum sativum and Spinach samples.
Quick Overview for Thylakoid Proteins antibody (ABIN165369)
Target
Thylakoid Proteins
Reactivity
Arabidopsis, Pisum sativum, Spinach
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Specificity
Higher plants, Synechocystis . Other photosynthetic organisms were not tested
Immunogen
extracted thylakoid proteins from spinach. Intact chloroplasts from spinach were isolated by isopycnic Percoll gradient centrifugation. Thylakoids were separated from stromal proteins after osmotic shock of chloroplasts. Thylakoids were washed in Tricine/Sucrose/MgCl 2 -containing buffer and used for rabbit immunisation. The thylakoid protein fraction contained thylakoid membrane and lumenal proteins.
Isotype
IgG
Application Notes
Western blot (ECL - 1 : 3.000 for higher plant proteins and 1: 1.000 for Synechocystis proteins)