P4HB antibody (AA 52-63)

Catalog No. ABIN1686617

The Mouse Monoclonal anti-P4HB antibody has been validated for WB, IHC, ELISA, IP, IF and ICC. It is suitable to detect P4HB in samples from Human.

Quick Overview for P4HB antibody (AA 52-63) (ABIN1686617)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 2E7-7

Product Details anti-P4HB Antibody

Binding Specificity: AA 52-63

Specificity: Detects ~48 kDa.

Cross-Reactivity: Human

Purification: Protein G Purified

Immunogen: Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1686617 was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI

Background: Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

Gene ID: 81567

NCBI Accession: NP_001139021

UniProt: Q8NBS9

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism