BAG3 antibody (Internal Region)

Catalog No. ABIN1686794

The Goat Polyclonal anti-BAG3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect BAG3 in samples from Human.

Quick Overview for BAG3 antibody (Internal Region) (ABIN1686794)

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-BAG3 Antibody

Binding Specificity: Internal Region

Purpose: BAG3

Sequence: KNAGNAEDPH TE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in Human Pancreas lysates (calculated MW of 61.6 kDa according to NP_004272.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BAG3

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Alternative Name: BAG3

Background: BAG3, BCL2-associated athanogene 3 , BAG-3, BIS, CAIR-1, MGC104307, BAG-family molecular chaperone regulator-3, BCL2-binding athanogene 3, Bcl-2-binding protein, docking protein CAIR-1

Molecular Weight: 70 kDa

Gene ID: 9531

NCBI Accession: NP_004272