pan Keratin antibody

Catalog No. ABIN966802

This Mouse Monoclonal antibody specifically detects pan Keratin in IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for pan Keratin antibody (ABIN966802)

Target: pan Keratin (panKRT)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B311-1

Product Details anti-pan Keratin Antibody

Specificity: This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.

Purification: Concentrated.

Immunogen: Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

Isotype: IgG1 kappa

Application Details

Application Notes: IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.

Comment:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.35 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Pan Cytokeratin, Pan CK-II

Molecular Weight: 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa