Cofilin antibody (pSer24) (AbBy Fluor® 350)

Catalog No. ABIN1693313

The Rabbit Polyclonal anti-Cofilin antibody has been validated for IF (cc) and IF (p). It is suitable to detect Cofilin in samples from Rat.

Quick Overview for Cofilin antibody (pSer24) (AbBy Fluor® 350) (ABIN1693313)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is conjugated to AbBy Fluor® 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cofilin Antibody (AbBy Fluor® 350)

Binding Specificity: pSer24

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CFL1/CFL2 around the phosphorylation site of Ser24

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin

Background:

Synonyms: Cofilin/CFL1 Ser24, 18 kDa phosphoprotein, CFL 1, CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, p18, COF1_HUMAN.

Background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH -dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Gene ID: 1072

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events