SP1 antibody (pThr453) (AbBy Fluor® 488)

Catalog No. ABIN1695013

This Rabbit Polyclonal antibody specifically detects SP1 in WB, IF (p) and IF (cc). It exhibits reactivity toward Mouse.

Quick Overview for SP1 antibody (pThr453) (AbBy Fluor® 488) (ABIN1695013)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-SP1 Antibody (AbBy Fluor® 488)

Binding Specificity: pThr453

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TSFP1 around the phosphorylation site of Thr453

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background:

Synonyms: SP1 Thr453, SP1 T453, p-SP1 phospho T453, p-TSFP1 phospho T453, Sp1 transcription factor isoform a, TSFP1, TSFP 1, Specicity protein 1, Transcription factor Sp1, SP 1, SP1, Sp1 transcription factor, SP1_HUMAN.

Background: Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A) (7), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.

Gene ID: 6667

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction