BCAR1 antibody (pTyr249) (AbBy Fluor® 555)

Catalog No. ABIN1696484

The Rabbit Polyclonal anti-BCAR1 antibody has been validated for IF (cc) and IF (p). It is suitable to detect BCAR1 in samples from Human.

Quick Overview for BCAR1 antibody (pTyr249) (AbBy Fluor® 555) (ABIN1696484)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is conjugated to AbBy Fluor® 555

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-BCAR1 Antibody (AbBy Fluor® 555)

Binding Specificity: pTyr249

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr249

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1

Background:

Synonyms: BCAR1 phospho Y249, BCAR1phospho Y249, p-BCAR1p-Tyr249, Breast cancer anti estrogen resistance 1 protein, BCAR 1, Bcar1, BCAR1_HUMAN, Breast cancer anti estrogen resistance 1, Breast cancer anti-estrogen resistance protein 1, CAS, Cas scaffolding protein family member 1, Crk associated substrate, Crk associated substrate p130Cas, CRK-associated substrate, CRKAS, P130CAS.

Background: p130 represents one of several known substrates for v-Crk encoded p47. p130 Cas (for Crk-associated substrate) exhibits a high level of tyrosine phosphorylation and is tightly associated with v-Crk, suggesting a role in v-Crk-mediated cell signaling. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.

Gene ID: 9564

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling