BNIP3 antibody (pSer95) (Biotin)

Catalog No. ABIN1700147

The Rabbit Polyclonal anti-BNIP3 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect BNIP3 in samples from Human.

Quick Overview for BNIP3 antibody (pSer95) (Biotin) (ABIN1700147)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BNIP3 Antibody (Biotin)

Binding Specificity: pSer95

Cross-Reactivity: Human

Predicted Reactivity: Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BNIP3 around the phosphorylation site of Ser95

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP3

Background:

Synonyms: BNIP3 phospho S95, p-BNIP3 phospho S95, BCL2 Adenovirus E1B 19 kDa Interacting Protein 3, BCL2/adenovirus E1B 19 kDa protein interacting protein 3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP 3, BNIP3, BNIP3_HUMAN, NIP 3, NIP3.

Background: The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL.

Gene ID: 664

Pathways: Autophagy, Brown Fat Cell Differentiation