ADH5 antibody (AA 301-374) (Biotin)

Catalog No. ABIN1700193

The Biotin-conjugated Rabbit Polyclonal anti-ADH5 antibody (ABIN1700193) specifically detects ADH5 in ELISA, IHC (p) and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for ADH5 antibody (AA 301-374) (Biotin) (ABIN1700193)

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH5 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ADH5 Antibody (Biotin)

Binding Specificity: AA 301-374

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ADH5

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ADH5

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Alternative Name: ADH5

Background:

Synonyms: ADH 3, ADH5, ADHX, ADHX_HUMAN, Alcohol dehydrogenase class III chi polypeptide, alcohol dehydrogenase 5 class III chi polypeptide, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class III, Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, class III alcohol dehydrogenase 5 chi subunit, FALDH, FDH, formaldehyde dehydrogenase, Glutathione dependent formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, hydroxymethyllutathione dehydrogenase, S-hydroxymethylglutathione dehydrogenase.

Background: The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including retinol, hydroxysteroids, ethanol, aliphatic alcohols and lipid peroxidation products. ADH5 (alcohol dehydrogenase 5 (class III)), also known as FDH (formaldehyde dehydrogenase), ADHX, ADH-3 or GSNOR, is a 374 amino acid cytoplasmic protein that belongs to the class III subfamily of alcohol dehydrogenases. Expressed ubiquitously, ADH5 uses iron as a cofactor to catalytically oxidize both long-chain primary alcohols and S-hydroxymethyl-glutathione, a product formed spontaneously between formaldehyde and glutathione. ADH5 exists as a homodimer and, via its ability to oxidize S-hydroxymethyl-glutathione and, thus, eliminate formaldehyde, functions as an important component of cellular metabolism. Genetic variations in the gene encoding ADH5 may affect drug and alcohol dependence in humans.

Gene ID: 128

UniProt: P11766