CSTF3 antibody (AA 151-250) (Biotin)

Catalog No. ABIN1700544

This anti-CSTF3 antibody is a Rabbit Polyclonal antibody detecting CSTF3 in WB, ELISA, IHC (p) and IHC (fro). Suitable for Mouse.

Quick Overview for CSTF3 antibody (AA 151-250) (Biotin) (ABIN1700544)

Target: CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTF3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CSTF3 Antibody (Biotin)

Binding Specificity: AA 151-250

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CSTF3/CSTF77

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CSTF3

Target: CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))

Alternative Name: CSTF3/CSTF77

Background:

Synonyms: CF 1 77 kDa subunit, Cleavage stimulation factor 77 kDa subunit, Cleavage stimulation factor subunit 3, Cleavage stimulation factor subunit 3 isoform 1, CSTF 3, CSTF 77, CSTF 77 kDa subunit, CSTF77, MGC117398, MGC43001, MGC75122, CSTF3_HUMAN.

Background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.

Gene ID: 1479