Rad23 (AA 51-150) antibody (Cy3)

Catalog No. ABIN1703454

The Cy3-conjugated Rabbit Polyclonal anti-Rad23 antibody (ABIN1703454) specifically detects Rad23 in WB, IF (cc) and IF (p). The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for Rad23 (AA 51-150) antibody (Cy3) (ABIN1703454)

Target: Rad23

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 51-150

Cross-Reactivity: Saccharomyces cerevisiae

Cross-Reactivity (Details): Saccharomyces cerevisiae

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae Rad23

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Rad23

Background:

Synonyms: DNA repair protein RAD23, UV excision repair protein RAD23, RAD23_YEAST, YEL037C, SYGP-ORF29.

Background: The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA (1). RAD23 resembles the other DNA repair genes, RAD2, RAD6, RAD7, RAD18, and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis (2). RAD23 encodes a nuclear protein containing a ubiquitin-like domain required for biological function (3). RAD23 bears a ubiquitin-like domain at its amino terminus and this ubiquitin-like domain affects protein function in a nonproteolytic manner (3). Rad23 is a highly conserved protein involved in nucleotide excision repair (NER) that associates with the proteasome via its amino-terminus (4). Its carboxy-terminal ubiquitin-associated domain is evolutionarily conserved from yeast to humans (4). In addition to a role in DNA repair events in yeast, several lines of evidence indicate that the Rad23 protein may regulate the activity of the 26 S proteasome (5).

Gene ID: 856674