Aconitase 1 antibody (pSer138) (Cy5)

Catalog No. ABIN1705082

The Rabbit Polyclonal anti-Aconitase 1 antibody has been validated for WB, IF (p) and IF (cc). It is suitable to detect Aconitase 1 in samples from Human.

Quick Overview for Aconitase 1 antibody (pSer138) (Cy5) (ABIN1705082)

Target: Aconitase 1 (ACO1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Aconitase 1 Antibody (Cy5)

Binding Specificity: pSer138

Predicted Reactivity: Human,Mouse,Rat,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Aconitase 1 around the phosphorylation site of Ser138 [AD(p-S)LQ]

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: Aconitase 1

Background:

Synonyms: Aconitase 1 phospho S138, p-Aconitase 1 phospho S138, ACO 1, ACO1, ACOC_HUMAN, Aconitase 1 soluble, Aconitase, Aconitase1, Aconitate hydratase, ACONS, Citrate hydro lyase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRE BP 1, IRE-BP 1, IREB 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, Iron responsive element binding protein 1, Iron-responsive element-binding protein 1, IRP 1, IRP1, OTTHUMP00000045233.

Background: Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins, iron regulatory protein-1 and -2 (IRP-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IREBP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide.

Gene ID: 48

Pathways: Transition Metal Ion Homeostasis