CYB5R3 antibody (Cy5.5)

Catalog No. ABIN1706219

The Rabbit Polyclonal anti-CYB5R3 antibody has been validated for WB. It is suitable to detect CYB5R3 in samples from Human, Mouse and Rat.

Quick Overview for CYB5R3 antibody (Cy5.5) (ABIN1706219)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is conjugated to Cy5.5

Application: Western Blotting (WB)

Product Details anti-CYB5R3 Antibody (Cy5.5)

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CYB5R3

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: Cyb5r3

Background:

Synonyms: B5R, Cyb5r3, Cytochrome b5 reductase 3, Cytochrome b5 reductase, DIA1, Diaphorase 1, Diaphorase-1, NADH cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, NB5R3_HUMAN, OTTHUMP00000028761, OTTHUMP00000198435, OTTHUMP00000198574, OTTHUMP00000198662, OTTHUMP00000198665.

Background: CYB5R3 is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism and, in erythrocytes, methemoglobin reduction. A serine residue at position 117 seems to only be found in persons of African origin. The allele frequency is 0.23 in African Americans. It is not found in Caucasians, Asians, Indo-Aryans or Arabs. This difference seems to have no effect on the enzyme activity. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1), in which the enzyme is only deficient in erythrocytes with a mild cyanosis, type 2 (HM2), in which the enzyme is completely deficient, and type 3 (HM3), where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied by mental retardation and neurological impairment.

Gene ID: 1727

Pathways: SARS-CoV-2 Protein Interactome