EIF1 antibody (AA 41-113) (Cy7)

Catalog No. ABIN1708171

This Rabbit Polyclonal antibody specifically detects EIF1 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for EIF1 antibody (AA 41-113) (Cy7) (ABIN1708171)

Target: EIF1 (Eukaryotic Translation Initiation Factor 1 (EIF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF1 antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-EIF1 Antibody (Cy7)

Binding Specificity: AA 41-113

Predicted Reactivity: Human,Mouse,Rat,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eIF1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF1

Target: EIF1 (Eukaryotic Translation Initiation Factor 1 (EIF1))

Alternative Name: eIF1

Background:

Synonyms: A121, e1, E1_HUMAN, E1A, Eukaryotic translation initiation factor 1, ISO1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1.

Background: In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.

Gene ID: 10209