ANAPC10 antibody (AA 31-130) (Cy7)

Catalog No. ABIN1708179

This anti-ANAPC10 antibody is a Rabbit Polyclonal antibody detecting ANAPC10 in WB, IF (cc) and IF (p). Suitable for Rat.

Quick Overview for ANAPC10 antibody (AA 31-130) (Cy7) (ABIN1708179)

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC10 antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ANAPC10 Antibody (Cy7)

Binding Specificity: AA 31-130

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APC10

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ANAPC10

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Alternative Name: APC10

Background:

Synonyms: ANAPC 10, anapc10, Anaphase promoting complex 10, Anaphase promoting complex subunit 10, Anaphase-promoting complex subunit 10, Apc 10, APC10, APC10_HUMAN, Cyclosome subunit 10, DKFZP564L0562, Doc 1, Doc1, OTTHUMP00000220297, OTTHUMP00000220298, OTTHUMP00000220299, OTTHUMP00000220300, OTTHUMP00000220303.

Background: Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis.

Gene ID: 10393

Pathways: M Phase