ANAPC10 antibody (N-Term)

Catalog No. ABIN2774722

This Rabbit Polyclonal antibody specifically detects ANAPC10 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for ANAPC10 antibody (N-Term) (ABIN2774722)

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ANAPC10 Antibody

Binding Specificity: N-Term

Purpose: ANAPC10 Antibody - N-terminal region

Sequence: MTTPNKTPPG ADPKQLERTG TVREIGSQAV WSLSSCKPGF GVDQLRDDNL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ANAPC10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANAPC10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43169-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ANAPC10 antibody (ABIN2774722)

Nourry, Maksumova, Pang, Liu, Wang: "Direct interaction between Smad3, APC10, CDH1 and HEF1 in proteasomal degradation of HEF1." in: BMC cell biology, Vol. 5, pp. 20, (2004) (PubMed).

Target Details for ANAPC10

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Alternative Name: ANAPC10

Background:

Targeted Subunit: 10

Background Information: ANAPC10 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and sub

Gene Name: Anaphase promoting complex subunit 10

Alternative Symbols: DOC1, APC10

Protein Name: Anaphase-promoting complex subunit 10

Molecular Weight: 21kDa

Gene ID: 10393

NCBI Accession: NP_055700

UniProt: Q9UM13

Pathways: M Phase