HPV16 E7 antibody (HRP)

Catalog No. ABIN1710867

This anti-HPV16 E7 antibody is a Rabbit Polyclonal antibody detecting HPV16 E7 in WB and IHC (p). Suitable for Human Papillomavirus 16 (HPV-16) and Virus.

Quick Overview for HPV16 E7 antibody (HRP) (ABIN1710867)

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Reactivity: Human Papillomavirus 16 (HPV-16), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPV16 E7 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HPV16 E7 Antibody (HRP)

Cross-Reactivity: Virus

Cross-Reactivity (Details): HPV16

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from HPV16 E7 protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HPV16 E7

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Alternative Name: HPV16 E7

Target Type: Viral Protein

Background:

Synonyms: E7, Human Papilloma Virus, Human papillomavirus type 16 E7, Human papillomavirus type 16 E7, Protein E7, Human Papillomavirus 16 E7, HPV16 E7, E7 protein HPV16, VE7_HPV16.

Background: Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90 % of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Gene ID: 1489079