APC antibody (pSer2054) (HRP)

Catalog No. ABIN1711113

This anti-APC antibody is a Rabbit Polyclonal antibody detecting APC in IHC (p) and IHC (fro). Suitable for Mouse.

Quick Overview for APC antibody (pSer2054) (HRP) (ABIN1711113)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APC Antibody (HRP)

Binding Specificity: pSer2054

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human APC around the phosphorylation site of Ser2054

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC

Background:

Synonyms: APC phospho S2054, p-APC phospho S2054, Adenomatous Polyposis Coli, APC, CC1, DP2, DP2.5, DP3, FAP, FPC, GS, Protein APC.

Background: Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Gene ID: 324

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization