Shevelled 2 (AA 421-520) antibody (HRP)

Catalog No. ABIN1711411

This Rabbit Polyclonal antibody specifically detects Shevelled 2 in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Rat.

Quick Overview for Shevelled 2 (AA 421-520) antibody (HRP) (ABIN1711411)

Target: Shevelled 2

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 421-520

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human shevelled 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Shevelled 2

Background:

Synonyms: Dishevelled 2 homologous to Drosophila dsh, Dishevelled dsh homolog 2, Dishevelled-2, Dishevelled2, DSH homolog 2, DVL 2, Dvl2, DVL2_HUMAN, Segment polarity protein dishevelled homolog DVL 2, Segment polarity protein dishevelled homolog DVL-2, Segment polarity protein dishevelled homolog DVL2.

Background: This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40 % amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq, Jul 2008].

Gene ID: 1856