Acetyl-CoA Carboxylase alpha antibody (pSer80) (HRP)

Catalog No. ABIN1711450

This Rabbit Polyclonal antibody specifically detects Acetyl-CoA Carboxylase alpha in WB, ELISA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Acetyl-CoA Carboxylase alpha antibody (pSer80) (HRP) (ABIN1711450)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase alpha antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Acetyl-CoA Carboxylase alpha Antibody (HRP)

Binding Specificity: pSer80

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Acetyl Coenzyme A Carboxylase alpha around the phosphorylation site of Ser80

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Alternative Name: Acetyl Coenzyme A Carboxylase alpha

Background:

Synonyms: Acetyl Coenzyme A Carboxylase alpha phospho S80, p-Acetyl Coenzyme A Carboxylase alpha phospho S80, ACAC, ACACA, ACACA, ACACA_HUMAN, ACC alpha, ACC, ACC-alpha, ACC1, ACC1, ACCA, acetyl CoA carboxylase 1, acetyl Coenzyme A, Acetyl Coenzyme A, Biotin carboxylase, Acetyl-Coenzyme A Carboxylase alpha.

Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect