MAEA antibody (AA 171-250) (HRP)

Catalog No. ABIN1711538

The Rabbit Polyclonal anti-MAEA antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect MAEA in samples from Human.

Quick Overview for MAEA antibody (AA 171-250) (HRP) (ABIN1711538)

Target: MAEA (Macrophage erythroblast Attacher (MAEA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAEA antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAEA Antibody (HRP)

Binding Specificity: AA 171-250

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EMP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAEA

Target: MAEA (Macrophage erythroblast Attacher (MAEA))

Alternative Name: MAEA/EMP/HLC10

Background:

Synonyms: 1110030D19Rik, Cell proliferation inducing gene 5 protein, Cell proliferation-inducing gene 5 protein, EMP, Erythroblast macrophage protein, HLC 10, HLC-10, HLC10, Human lung cancer oncogene 10 protein, Lung cancer related protein 10, lung cancer-related protein 10, Macrophage erythroblast attacher, MAEA, MAEA_HUMAN, MGC93683, PIG5, proliferation-inducing gene 5.

Background: Emp is a 396 amino acid ubiquitously expressed adhesion protein. Expressed as 5 alternatively spliced isoforms, Emp contains one CTLH domain and one LisH domain. Emp can form a complex with F-actin, which is involved regulating actin distribution in erythroblasts and macrophages. Considered to assist with cell division and nuclear architecture, Emp is localized with condensed chromatin at prophase, nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. Although the exact function of Emp is unknown, Emp is suggested to be involvement in erythroblast-macrophage cell attachment, terminal maturation and enucleation of erythroid cells, and inhibiting apoptosis of erythroblasts.

Gene ID: 10296