Cofilin antibody (pSer24) (HRP)

Catalog No. ABIN1711597

This Rabbit Polyclonal antibody specifically detects Cofilin in ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for Cofilin antibody (pSer24) (HRP) (ABIN1711597)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cofilin Antibody (HRP)

Binding Specificity: pSer24

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CFL1/CFL2 around the phosphorylation site of Ser24

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin

Background:

Synonyms: Cofilin/CFL1 Ser24, 18 kDa phosphoprotein, CFL 1, CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, p18, COF1_HUMAN.

Background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH -dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Gene ID: 1072

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events