Dynamin 1 antibody (AA 551-680) (HRP)

Catalog No. ABIN1711604

The Rabbit Polyclonal anti-Dynamin 1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Dynamin 1 in samples from Human.

Quick Overview for Dynamin 1 antibody (AA 551-680) (HRP) (ABIN1711604)

Target: Dynamin 1 (DNM1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Dynamin 1 Antibody (HRP)

Binding Specificity: AA 551-680

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dynamin 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin 1

Background:

Synonyms: B dynamin, D100, DNM 1, DNM, DNM1, DYN1_HUMAN, Dynamin, Dynamin-1, Dynamin1.

Background: Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles (1). Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (25). The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, which is located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase (25). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that phosphorylation affects the subcellular localization of Dynamin I (5). Mouse, rat and human Dynamin I are phosphorylated on serine residues, including Ser 778, by Cdk5, regulating PACSIN1 recruitment and enabling synaptic vesicle endocytosis.

Gene ID: 1759

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling